Research Overview

Dr. Boulias is interested in the emerging field of epitrancriptomics, which encompasses the biology of RNA modifications and their associated factors. He is focused on determining the genetic and molecular basis of the control of gene expression by specific mRNA modifications. In particular, he investigates the functions of N6-methyladenosine in RNA biology in response to physiological and environmental stimuli using a variety of biochemical and cell biology tools in cell lines and murine experimental models. His ultimate goal is to identify new players and novel mechanisms of RNA metabolism that can be utilized as targets of therapeutic action for the treatment of related diseases.

Research Background

Dr. Boulias received his PhD degree from University of Crete, Greece. He completed his postdoctoral training at MIT and then moved to BCH as Senior Research Associate. In 2018 he was appointed as Instructor in Pediatrics at BCH and HMS.

Publications

  1. 18S rRNA methyltransferases DIMT1 and BUD23 drive intergenerational hormesis. Mol Cell. 2023 09 21; 83(18):3268-3282.e7. View Abstract
  2. Paternal methotrexate exposure affects sperm small RNA content and causes craniofacial defects in the offspring. Nat Commun. 2023 03 23; 14(1):1617. View Abstract
  3. Biological roles of adenine methylation in RNA. Nat Rev Genet. 2023 03; 24(3):143-160. View Abstract
  4. Means, mechanisms and consequences of adenine methylation in DNA. Nat Rev Genet. 2022 07; 23(7):411-428. View Abstract
  5. The adenine methylation debate. Science. 2022 02 04; 375(6580):494-495. View Abstract
  6. Methylation of viral mRNA cap structures by PCIF1 attenuates the antiviral activity of interferon-ß. Proc Natl Acad Sci U S A. 2021 07 20; 118(29). View Abstract
  7. Coping with darkness: The adaptive response of marine picocyanobacteria to repeated light energy deprivation. Limnol Oceanogr. 2021 Sep; 66(9):3300-3312. View Abstract
  8. Detection of DNA Methylation in Genomic DNA by UHPLC-MS/MS. Methods Mol Biol. 2021; 2198:79-90. View Abstract
  9. Adenine DNA methylation, 3D genome organization, and gene expression in the parasite Trichomonas vaginalis. Proc Natl Acad Sci U S A. 2020 06 09; 117(23):13033-13043. View Abstract
  10. N6-adenosine methylation of ribosomal RNA affects lipid oxidation and stress resistance. Sci Adv. 2020 04; 6(17):eaaz4370. View Abstract
  11. Identification of the m6Am Methyltransferase PCIF1 Reveals the Location and Functions of m6Am in the Transcriptome. Mol Cell. 2019 08 08; 75(3):631-643.e8. View Abstract
  12. Put the Pedal to the METTL1: Adding Internal m7G Increases mRNA Translation Efficiency and Augments miRNA Processing. Mol Cell. 2019 06 20; 74(6):1105-1107. View Abstract
  13. Sources of artifact in measurements of 6mA and 4mC abundance in eukaryotic genomic DNA. BMC Genomics. 2019 Jun 03; 20(1):445. View Abstract
  14. An Epigenetic Clock Measures Accelerated Aging in Treated HIV Infection. Mol Cell. 2016 04 21; 62(2):153-155. View Abstract
  15. The C. elegans microRNA mir-71 acts in neurons to promote germline-mediated longevity through regulation of DAF-16/FOXO. Cell Metab. 2012 Apr 04; 15(4):439-50. View Abstract
  16. 3-Ketoacyl thiolase delays aging of Caenorhabditis elegans and is required for lifespan extension mediated by sir-2.1. Proc Natl Acad Sci U S A. 2010 Nov 02; 107(44):18927-32. View Abstract
  17. Proteomic Analysis of Liver from Transgenic Mice Overexpressing Small Heterodimer Partner. Cancer Genomics Proteomics. 2006 Mar-Apr; 3(2):119-126. View Abstract
  18. Regulation of hepatic metabolic pathways by the orphan nuclear receptor SHP. EMBO J. 2005 Jul 20; 24(14):2624-33. View Abstract
  19. Functional role of G9a-induced histone methylation in small heterodimer partner-mediated transcriptional repression. Nucleic Acids Res. 2004; 32(20):6096-103. View Abstract
  20. Synergism between nuclear receptors bound to specific hormone response elements of the hepatic control region-1 and the proximal apolipoprotein C-II promoter mediate apolipoprotein C-II gene regulation by bile acids and retinoids. Biochem J. 2003 Jun 01; 372(Pt 2):291-304. View Abstract
  21. Cross-talk between catalytic and regulatory elements in a DEAD motor domain is essential for SecA function. EMBO J. 2001 Mar 01; 20(5):961-70. View Abstract

Contact Konstantinos Boulias

Phone: 617-919-6974
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